human tl1a elisa kits  (R&D Systems)

Journal: bioRxiv

Article Title: Eicosatetraynoic Acid Regulates Pro-Fibrotic Pathways in an Induced Pluripotent Stem Cell Derived Macrophage:Human Intestinal Organoid Model of Crohn’s Disease

doi: 10.1101/2024.01.30.577959

Figure Lengend Snippet: iPSC lines A, B, C, and D derived from PBMC obtained from four pediatric-onset CD patients were differentiated into macrophages. iPSC-derived macrophages were assayed under basal conditions or following LPS (100 ng/mL for 72 hours) stimulation. Cytokine and chemokine secretion was measured using a custom luminex assay or ELISA. Data are shown as the mean (SEM), n=6-12 per group, *p<0.05, ***p<0.001, ****p<0.0001.

Article Snippet: CCL2 and TL1A were measured using human CCL2 (MCP-1) and human TL1A ELISA kits by R&D Systems (R&D Systems, Minneapolis, MN).

Techniques: Derivative Assay, Luminex, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Eicosatetraynoic Acid Regulates Pro-Fibrotic Pathways in an Induced Pluripotent Stem Cell Derived Macrophage:Human Intestinal Organoid Model of Crohn’s Disease

doi: 10.1101/2024.01.30.577959

Figure Lengend Snippet: iPSC-derived macrophages were assayed under basal conditions or following LPS (100 ng/mL for 72 hours) ± ETYA (50 μM for 72 hours) stimulation. Cytokine and chemokine secretion was measured using a custom luminex assay or ELISA. Data are shown as the mean (SEM), n=18-21 per group, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: CCL2 and TL1A were measured using human CCL2 (MCP-1) and human TL1A ELISA kits by R&D Systems (R&D Systems, Minneapolis, MN).

Techniques: Derivative Assay, Luminex, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Increased Serum Levels of Tumor Necrosis Factor-like Ligand 1A in Atopic Dermatitis.

doi: 10.3390/ijms24031813

Figure Lengend Snippet: Figure 1. Serum TL1A protein levels in patients with AD and normal controls. The measured values from individual patients were plotted by dots. Each bar means the average. ** p < 0.01. (a) Serum TL1A protein levels in AD (n = 36) or normal controls (n = 10). (b) Serum TL1A levels in mild (n = 14) or severe (n = 22) AD patients and normal controls (n = 16).

Article Snippet: Serum TL1A levels were quantified using Human TL1A ELISA kit (DY1319–05 and DY008, R&D Systems, Minneapolis, MN, USA).

Techniques:

Journal: International journal of molecular sciences

Article Title: Increased Serum Levels of Tumor Necrosis Factor-like Ligand 1A in Atopic Dermatitis.

doi: 10.3390/ijms24031813

Figure Lengend Snippet: Figure 2. Correlation between TL1A protein levels and clinical markers in TL1A positive patients of AD. (a) Correlation between serum TL1A protein levels and Eczema Area and Severity Index (EASI). (b) Correlation between serum TL1A levels and the age of each patient. (c) Correlation between serum TL1A levels and serum thymus and activation-regulated chemokine (TARC) levels. (d) Correlation between serum TL1A levels and serum immunoglobulin E (IgE) levels. (e) Correlation between serum TL1A levels and serum lactate dehydrogenase (LDH) levels. (f) Correlation between serum TL1A levels and the number of eosinophils in peripheral blood.

Article Snippet: Serum TL1A levels were quantified using Human TL1A ELISA kit (DY1319–05 and DY008, R&D Systems, Minneapolis, MN, USA).

Techniques: Activation Assay

Journal: International journal of molecular sciences

Article Title: Increased Serum Levels of Tumor Necrosis Factor-like Ligand 1A in Atopic Dermatitis.

doi: 10.3390/ijms24031813

Figure Lengend Snippet: Figure 4. Immunohistochemistry of TL1A in atopic dermatitis (AD) or normal skin samples.

Article Snippet: Serum TL1A levels were quantified using Human TL1A ELISA kit (DY1319–05 and DY008, R&D Systems, Minneapolis, MN, USA).

Techniques: Immunohistochemistry

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: TL1A-DR3 plasma levels are predictive of HIV-1 disease control, and DR3 co-stimulation boosts HIV-1-specific T-cell responses

doi: 10.4049/jimmunol.2000933

Figure Lengend Snippet: A–B) Correlation between plasma DR3 protein levels and the percentage of effector CD8 T cells (A) and HLA-DR+ CD45RO+ CD8 T cells (B) in a cohort of treated individuals (n=26, Table I). C) Correlation between ELISA-determined plasma TL1A levels and the percentage of CD25high FOXP3+ T cells (circles CD4 T cells and squares CD8 T cells) in treated individuals including immune-concordant and -discordant individuals (n=26, Table I). The Spearman rank test was used for the correlations, and p-values <0.05 were considered statistically significant.

Article Snippet: Cytokine determination by ELISA: TL1A was determined using an ELISA kit (Human TL1A/TNFSF15 DuoSet ELISA, R&D Systems).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: TL1A-DR3 plasma levels are predictive of HIV-1 disease control, and DR3 co-stimulation boosts HIV-1-specific T-cell responses

doi: 10.4049/jimmunol.2000933

Figure Lengend Snippet: sTL1A (A) and sDR3 (B) relative plasma levels (Z-score “communicome” values) in HIV-High (n=47) and HIV-Low (n=49) (Table S1). C-D) Gene expression (Table S1) of TL1A (C) and DR3 (D) in HIV-High (n=16) and HIV-Low (n=30). E-F) Unrelated validation cohorts for gene expression of TL1A (E) and DR3 (F) in dry pellet PBMC samples from seronegatives (SN, n=6) and HIV-infected individuals one year before and after initiation of treatment (untreated [n=11] and treated [n=5]) and in LTNP (n=23) (Table S1). G) Absolute DR3 quantifications in plasma (ELISA) in a confirmatory cohort including seronegatives (SN, n=8) and samples from time points one year before and after initiation of treatment (untreated [n=15] and treated [n=10]) and controllers (n=31), Table S1). The Mann-Whitney test was applied for group comparisons, and p-values < 0.05 were considered significant.

Article Snippet: Cytokine determination by ELISA: TL1A was determined using an ELISA kit (Human TL1A/TNFSF15 DuoSet ELISA, R&D Systems).

Techniques: Clinical Proteomics, Gene Expression, Biomarker Discovery, Infection, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY